New Step by Step Map For Explore Ligaz Options
New Step by Step Map For Explore Ligaz Options
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This case invites the design of C-2-substituted analogs of adenosine nucleotides and NAD+ as selective inhibitors of LigA. Certainly, it was noted not too long ago that 2-methylthio-ATP is really a potent inhibitor of the adenylylation response of E. coli
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The undifferentiated nucleotide specificities of specified archaeal ligases have tantalizing implications for enzyme evolution and also the probable utilization of ADP as being a substrate by ancestors of contemporary DNA ligases. The ability of some archaeal ligases to make the most of ATP and NAD+ or ATP and ADP as substrates could be explained when they identified just the ADP component widespread to all 3 nucleotides.
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